Phages were recommended as promising biocontrol brokers in meals, however trials demonstrating the potency of phage remedy below commercial settings are lacking. Right here we carried out a full-scale commercial trial to guage the efficacy of a business phage product to scale back the superiority of naturally going on Salmonella on red meat carcasses. A complete of 134 carcasses from probably Salmonella-positive finisher herds have been selected to be examined on the slaughterhouse in keeping with the extent of antibodies within the blood. All the way through 5 consecutive runs, carcasses have been directed right into a cabin spraying phages, leading to a dosage of roughly 2 × 107 phages in step with cm2 carcass floor. To judge the presence of Salmonella, a predefined space of 1 part of the carcass used to be swabbed ahead of phage software and the opposite part 15 min after. A complete of 268 samples have been analysed by way of Actual-Time PCR.
Below those optimized take a look at prerequisites, 14 carcasses have been discovered effective ahead of phage software, whilst simplest 3 carcasses have been effective after.
This paintings presentations that phage software permits to reach approximatively 79% aid of Salmonella-positive carcasses and demonstrates that implementation of phage software in commercial settings can be utilized as an extra method to keep watch over foodborne pathogens.
Volpi M, Gambino M, Kirkeby Okay, Elsser-Gravesen A, Brøndsted L.
Complete-scale commercial phage trial concentrated on Salmonella on red meat carcasses. Meals Microbiology. 2023; 112: 104240. https://doi.org/10.1016/j.fm.2023.104240.